Hygromycin B
Hygromycin B|潮霉素B|蛋白质合成抑制剂|罗氏
中文全名: 潮霉素 B
英文全名:
别 名: Hygromix
分 子 式: C20H37N3O13
分 子 量: 527.52
外 观: 橙色溶液
说 明: 蛋白质合成抑制剂,可用于植物细胞培养等生化研究
特 性: 原液不要冷冻,否则可能失活。稀释后,保存时间将大大降低,应尽快用完
储存条件: 2-8 ℃
潮霉素B,
Roche 货号:10843555001
其他品牌(潮霉素B Amresco 货号:amresco J607)
from Streptomyces hygroscopicus. Antibiotic solution for selection of hygromycin resistance in transfected cells, filtered through 0.2 µm pore size membrane.
Catalog # | Pack size | Qty | Price | Options |
10843555001 | 1 g (20 ml) filtered through 0.2 µm pore size membrane | Please contact us for purchase. |
Application
is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).
For life science research only.
Product Description
Formula:
C20H37N3O13
LD50:
6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
Molecular weight:
Mr = 527.5
Working concentration:
50 - 1,000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.
Contents
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 µm pore size membrane
Quality
Purity:
>80% (HPLC)