更新时间：2014-04-08 
阅读：2578R&D Systems公司 IL-1 beta（白介素1 beta）检测试剂盒，现货抢购！ 货号：MLB00C
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Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:4.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity:4.8 pg/mL
Assay Range:12.5 - 800 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma)
Specificity:Natural and recombinant mouse IL-1 beta
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference:No significant interference observed with available related molecules.
Product Summary:
The Quantikine Mouse IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 beta in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accuray. Results obtained using natural mouse IL-1 beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 beta.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 33.5 | 92.9 | 386 | 34.6 | 93.5 | 423 |
| Standard Deviation | 2.5 | 4.3 | 11.7 | 2.9 | 6.2 | 23.9 |
| CV% | 7.5 | 4.6 | 3 | 8.4 | 6.6 | 5.7 |
Recovery:
The recovery of mouse IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 106 | 95-119 |
| Cell Lysates (n=4) | 115 | 110-120 |
| Serum (n=4) | 105 | 98-113 |
| EDTA Plasma (n=4) | 108 | 101-117 |
| Heparin Plasma (n=4) | 97 | 87-114 |
Linearity:
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-1 beta were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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